By Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof
Environmental matters concerning non-stop use of man-made dyes observed a revival within the call for for ordinary dyes as typical dyes express greater biodegradability and usually have a better compatibility with the surroundings. in spite of the fact that, one of many obstacles at the use of ordinary dyes or pigments is the low extraction yield components (a few grams of pigment consistent with kg of dried uncooked material). hence, the exploitation of alternative organic assets akin to fungi, micro organism and phone cultures deals attention-grabbing replacement. Microbial pigments akin to from bacterial origins supply the virtue when it comes to construction in comparison to pigments extracted from greens or animals, as a result of its basic telephone and quick culturing approach. This publication bargains fascinating perception into preliminary works conducted to illustrate the aptitude use of bacterial pigment as colorant for varied applications.
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Additional info for Application of Bacterial Pigments as Colorant: The Malaysian Perspective
The antibacterial activity of a material depends on the destruction of the physical structure or the inhibition of reaction in a microorganism; it seems that the presence and the level of the antibacterial activity of the pigment varied significantly with the type of microorganism used (Martinko and Madigan 2006). It can be seen from Fig. 14 that the yellow pigment has some antimicrobial activity as evidenced from the clearing zones observed in the plates containing Bacillus cereus and Escherichia coli.
A. 1007/978-3-642-24520-6_3, Ó The Author(s) 2012 45 46 3 Optimization of Pigment Production Fig. 1 Growth profile of C. violaceum in NB distilled water in a 1 L volumetric flask. 45 lm Whatman filter paper. Growth Profile of C. violaceum: A 12 h-grown culture of bacteria (20 mL) was inoculated into a 2 L Erlenmeyer flask containing 180 mL of NB followed by incubation at 200 rpm, 30 8C for 24 h. Culture turbidity (OD600) was measured at regular intervals using a spectrophotometer (Genesys 20, ThermoSpectronic).
Coli; (a) - Control (bacteria only); (b) - bacteria ? methanol (medium suspension for pigment); (c) - bacteria ? methanol ? violet pigment Similar experimental setup consisting of filter paper discs impregnated with 50 lL methanol and filter paper discs only acted as control. The appearance of the inhibition zone on both the Gram-positive (S. aureus and B. cereus) and the Gram-negative bacteria (P. aeruginosa and E. coli) indicated that the violet pigment has a broadspectrum antibacterial property (August et al.
Application of Bacterial Pigments as Colorant: The Malaysian Perspective by Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof